| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Suínos e Aves. |
|
Data corrente: |
20/08/2002 |
|
Data da última atualização: |
02/08/2013 |
|
Autoria: |
PAIVA, D. P. de. |
|
Afiliação: |
Embrapa Suínos e Aves. |
|
Título: |
Controle integrado de moscas em avicultura intensiva de postura. |
|
Ano de publicação: |
2001 |
|
Fonte/Imprenta: |
Revista da AVIMIG, v.1, n.6, p.16-17, 2001. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Controle de moscas. |
|
Thesagro: |
Avicultura. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00378naa a2200133 a 4500
001 1439819
005 2013-08-02
008 2001 bl --- 0-- u #d
100 1 $aPAIVA, D. P. de
245 $aControle integrado de moscas em avicultura intensiva de postura.
260 $c2001
650 $aAvicultura
653 $aControle de moscas
773 $tRevista da AVIMIG$gv.1, n.6, p.16-17, 2001.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
10/05/2022 |
|
Data da última atualização: |
10/05/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; ALMEIDA, N. N. C.; FIGUEIRO, M. R.; QUINTAO, C. C. R.; GARCIA, J. M. |
|
Afiliação: |
NAIARA ZOCCAL SARAIVA, CNPGL; CLARA SLADE OLIVEIRA, CNPGL; NATHALIA N. C. ALMEIDA, Universidade Federal do Pará; MARIVALDO RODRIGUES FIGUEIRO, CPATU; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAQUIM M. GARCIA, Universidade Estadual Paulista. |
|
Título: |
Epigenetic modifiers during in vitro maturation as a strategy to increase oocyte competence in bovine. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Theriogenology, v. 187, p. 95-101, 2022. |
|
DOI: |
https://doi.org/10.1016/j.theriogenology.2022.04.014 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Oocyte in vitro maturation (IVM) is a key procedure for the in vitro production (IVP) of bovine embryos; however, the efficiency of this step is limited by the intrinsic developmental competence of oocytes. This study aimed to investigate possible epigenetic changes resulting from using of histone deacetylase (HDAC) inhibitors in the maturation of bovine oocytes and subsequent embryo development. Initially, we investigated the meiotic progression of bovine oocytes matured in vitro in the presence of trichostatin A (TSA). We then evaluated histone H3k9 acetylation levels in oocytes exposed to different TSA concentrations, and the relative expression of genes linked to oocyte competence. Finally, we studied preimplantation embryonic development by analyzing the cleavage, blastocysts, and hatching rates. Acetylation levels of H3k9 increased (p < 0.05) when oocytes were exposed to 50 nM or 100 nM TSA during IVM, but there were no significant changes in the relative expression of the evaluated genes p34cdc2, cyclin B1, MAPK, GDF9, G6PDH, and HSP70. We found that 5 nM TSA promoted the attenuation of meiotic progression and positively affected pre-implantation embryo development in bovine species, allowing a 10% increase in the blastocyst rate. We concluded that TSA treatment during IVM was efficient in promoting changes in H3k9 acetylation levels from 50 nM and promoted attenuated meiotic progression in bovine oocytes at all concentrations evaluated, with a positive impact on pre-implantation development when used at low concentrations. MenosOocyte in vitro maturation (IVM) is a key procedure for the in vitro production (IVP) of bovine embryos; however, the efficiency of this step is limited by the intrinsic developmental competence of oocytes. This study aimed to investigate possible epigenetic changes resulting from using of histone deacetylase (HDAC) inhibitors in the maturation of bovine oocytes and subsequent embryo development. Initially, we investigated the meiotic progression of bovine oocytes matured in vitro in the presence of trichostatin A (TSA). We then evaluated histone H3k9 acetylation levels in oocytes exposed to different TSA concentrations, and the relative expression of genes linked to oocyte competence. Finally, we studied preimplantation embryonic development by analyzing the cleavage, blastocysts, and hatching rates. Acetylation levels of H3k9 increased (p < 0.05) when oocytes were exposed to 50 nM or 100 nM TSA during IVM, but there were no significant changes in the relative expression of the evaluated genes p34cdc2, cyclin B1, MAPK, GDF9, G6PDH, and HSP70. We found that 5 nM TSA promoted the attenuation of meiotic progression and positively affected pre-implantation embryo development in bovine species, allowing a 10% increase in the blastocyst rate. We concluded that TSA treatment during IVM was efficient in promoting changes in H3k9 acetylation levels from 50 nM and promoted attenuated meiotic progression in bovine oocytes at all concentrations evaluated, with a positive impact on pre-... Mostrar Tudo |
|
Palavras-Chave: |
Cromatina; Epigenética; Oocito; Tricostatina A. |
|
Thesagro: |
Bovino; Embrião; Reprodução Animal. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02377naa a2200277 a 4500
001 2142833
005 2022-05-10
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2022.04.014$2DOI
100 1 $aSARAIVA, N. Z.
245 $aEpigenetic modifiers during in vitro maturation as a strategy to increase oocyte competence in bovine.$h[electronic resource]
260 $c2022
520 $aOocyte in vitro maturation (IVM) is a key procedure for the in vitro production (IVP) of bovine embryos; however, the efficiency of this step is limited by the intrinsic developmental competence of oocytes. This study aimed to investigate possible epigenetic changes resulting from using of histone deacetylase (HDAC) inhibitors in the maturation of bovine oocytes and subsequent embryo development. Initially, we investigated the meiotic progression of bovine oocytes matured in vitro in the presence of trichostatin A (TSA). We then evaluated histone H3k9 acetylation levels in oocytes exposed to different TSA concentrations, and the relative expression of genes linked to oocyte competence. Finally, we studied preimplantation embryonic development by analyzing the cleavage, blastocysts, and hatching rates. Acetylation levels of H3k9 increased (p < 0.05) when oocytes were exposed to 50 nM or 100 nM TSA during IVM, but there were no significant changes in the relative expression of the evaluated genes p34cdc2, cyclin B1, MAPK, GDF9, G6PDH, and HSP70. We found that 5 nM TSA promoted the attenuation of meiotic progression and positively affected pre-implantation embryo development in bovine species, allowing a 10% increase in the blastocyst rate. We concluded that TSA treatment during IVM was efficient in promoting changes in H3k9 acetylation levels from 50 nM and promoted attenuated meiotic progression in bovine oocytes at all concentrations evaluated, with a positive impact on pre-implantation development when used at low concentrations.
650 $aBovino
650 $aEmbrião
650 $aReprodução Animal
653 $aCromatina
653 $aEpigenética
653 $aOocito
653 $aTricostatina A
700 1 $aOLIVEIRA, C. S.
700 1 $aALMEIDA, N. N. C.
700 1 $aFIGUEIRO, M. R.
700 1 $aQUINTAO, C. C. R.
700 1 $aGARCIA, J. M.
773 $tTheriogenology$gv. 187, p. 95-101, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
